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I think that out of all 3 actives you stated, Retinol alone has been around long enough to warrant effectivity in long term use. Actually retinol is the OTC version of tretinoin/retinoic acid and is generally the milder formula. Tretinoin in comes in different formulations = .1%, .05%, and .025% and the higher the formualtion, the more effective, but also the greater the irritation. Tretinoin functions through exfoliation and increasing collagen production (within 3 months use), thereby decreasing and preventing wrinkles at the same time. But it has to be used every 2 days at least, to be effective. Also maintenance treatment is required. Higher concentrations of AHA (15% and up) have also been proven to increase collagen production. So this is your best bet if you're pregnant or wary of using tretinoin.The Holy grail of anti-aging remains: sunscreen + retinoids + antioxidants (topical vitamin c)Also, I must note that Botox does not increase collagen production, it simply freezes the muscles so as to prevent the skin from forming permanent lines, that are induced by facial expressions.